siRNA profiling of human aortic vascular smooth muscle cells knocked down for FoxM1
ID:
状态:
发布时间Sept. 6, 2009
, 更新时间 May 1, 2014
, 提交时间 Aug. 6, 2009,
物种:
Homo sapiens
摘要:
Transcription factor FoxM1 is expressed in proliferating cells, and its expression is critical for cell proliferation in embryos and tumors. FoxM1 regulates a multi-gene transcriptional network for cell cycle regulation. We used microarrays to detail the global program of gene expression either directly or indirectly regulated by FoxM1, and distinct classes of up- and down-regulated genes. Experiment Overall Design: Human aortic vascular smooth muscle cells (HAVSMC) were transfected with FoxM1 siRNA and control siRNA for 48 hours. Total RNA was extracted, purified, and prepared for hybridization on Affymetrix microarrays.