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题目:: Cj1223c Mutant vs. Overexpressed, Time Course
ID:
状态:: 发布时间Aug. 26, 2005 , 更新时间 Oct. 18, 2011 , 提交时间 Aug. 24, 2005,
物种:: Campylobacter jejuni
摘要:: The Cj1223c gene was cloned downstream of a strong promoter into the replicating plasmid pRY108, and was highly expressed in the wild type Campylobacter jejuni 81-176 strain (overexpressed). The Cj1223c gene was knocked out by allelic replacement in the 81-176 strain background (mutant). This mutant also carried an empty pRY108 vector. The Cj1223c overexpressing strain and the mutant were grown overnight in liquid broth, supplemented with kanamycin. The following morning, both cultures were diluted back to O.D.600=0.100, and allowed to continue to grow, shaking. At various time intervals (2, 6, 10, and 24 hours), samples of each culture were removed, combined with 1/10 stop solution, and the bacteria pelleted for RNA preparation. An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract. Computed
实验种类:: unknown experiment type
样本量:: 16
实验设计:
: 无设计数据
数据号:: E-GEOD-3198, GSE3198
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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