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题目:: Fibroblast-like synoviocytes derived from patients with rheumatoid arthritis
ID:
状态:: 发布时间Jan. 18, 2006 , 更新时间 March 27, 2012 , 提交时间 Jan. 18, 2006,
物种:: Homo sapiens
摘要:: Fibroblast-like synoviocytes (FLS) were cultured from synovial tissues from 19 RA patients. RNA was isolated using the Fast Track 2.0 kit (Invitrogen, Carlsbad, California)from the FLS cultures. Fluorescent cDNA probes were prepared from 1 ug experimental mRNA by oligo dT-primed polymerization using Superscript II reverse transcriptase in the presence of Cy5 labeled dCTP. A common reference mRNA sample (CRE) that consists of a mixture of mRNAs isolated from 11 different cell-lines, was labeled with Cy3. The Cy5 and Cy3 labeled cDNAs were pooled and hybridized to a 24,000 cDNA microarray. Set of arrays that are part of repeated experiments Biological Replicate Using regression correlation
实验种类:: unknown experiment type
样本量:: 38
实验设计:
: 无设计数据
数据号:: E-GEOD-4061, GSE4061
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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