Transcription profiling of mouse pancreatic duct cell lines infected with Ngn3-expressing adenovirus

We have developed an in vitro model of Ngn3-dependent differentiation by infecting pancreatic duct cell lines with an Ngn3-expressing adenovirus. We used glass microarrays containing 18,000 mouse cDNAs and compared gene expression patterns in mPAC L20 cells infected with either AdCMV-gal (control) or AdCMV-ngn3. All comparisons were performed 48 h after viral infection, meaning that genes differentially expressed in Ngn3-expressing mPAC cells could include genes whose expression was directly or indirectly controlled by Ngn3.