ChIP-Seq sequencing of novel interactors of H3K4me3, H3K36me3 and H3K9me3

·Released Sept. 17, 2010 ·Updated April 3, 2013

Samples

Assays

References

Description

This study describes the epigenetic profiling of the novel interactors of H3K4me3, H3K36me3 or H3K9me3. The interactors were ChIP-Seq profiled by their GFP tag in stably transfected HeLa (Kyoto) cells. The interactors include GATAD1, Sgf29, BAP18, TRRAP, PHF8, N-PAC and LRWD1 (including replicates), as well as an GFP ChIP-Seq profile on non-transfected HeLa cells (negative control). Also included are the profiles of the histone modifications themselves (H3K4me3, H3K27me3, H3K9me3, H3K36me3, H3K9/14Ac and H3K79me3) ChIP-Seq profiling of 8 proteins by their GFP tag in stably transfected cells HeLa (Kyoto) cells, 6 replicas, as well as ChIP-Seq profiling of 6 histone modifications in wt HeLa (Kyoto) cells

References

Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers.

Vermeulen M, Eberl HC, Matarese F, Marks H, Denissov S, Butter F, Lee KK, Olsen JV, Hyman AA, Stunnenberg HG, Mann M

PMID: 20850016

Sample Attributes

antibody

anti-GFP, anti-H3K27me3, anti-H3K36me3, anti-H3K4me3, anti-H3K79me3, anti-H3K9/14Ac, anti-H3K9me3

antibody manufacturer

Abcam, custom GFP antibody generously provided by Ina Poser, custom GFP from Ina Poser, Diagenode, Upstate/Millipore

cell line

HeLa Kyoto cell line

Organism

Homo sapiens

sample type

Antibody GFP (custom GFP antibody generously provided by Ina Poser); HeLa cells stably transfected with GATAD1-GFP BAC, Antibody GFP (custom GFP antibody generously provided by Ina Poser); HeLa cells stably transfected with N-PAC-GFP BAC, Antibody GFP Abcam (ab290-050), Antibody GFP Abcam (ab290-050); HeLa cells stably transfected with BAP18-GFP BAC, Antibody GFP Abcam (ab290-050); HeLa cells stably transfected with GATAD1-GFP BAC, Antibody GFP Abcam (ab290-050); HeLa cells stably transfected with LRWD1-GFP BAC, Antibody GFP Abcam (ab290-050); HeLa cells stably transfected with N-PAC-GFP BAC, Antibody GFP Abcam (ab290-050); HeLa cells stably transfected with PHF8-GFP BAC, Antibody GFP Abcam (ab290-050); HeLa cells stably transfected with Sgf29-GFP BAC, Antibody GFP Abcam (ab290-050); HeLa cells stably transfected with TRRAP-GFP BAC, Antibody H3K27me3 from Upstate/Millipore (07-449), Antibody H3K36me3 from Diagenode (CS-058-100), Antibody H3K4me3 Diagenode (pAb-MEHAHS-024), Antibody H3K79me3 from Abcam (ab2621), Antibody H3K9/14Ac from Diagenode (pAb-ACHBHS-044), Antibody H3K9me3 from Abcam (Ab8898-100)

transfection

None, BAP18-GFP BAC, GATAD1-GFP BAC, LRWD1-GFP BAC, N-PAC-GFP BAC, PHF8-GFP BAC, Sgf29-GFP BAC, TRRAP-GFP BAC

Experiment Info

Accession

E-GEOD-20303

GEO ID

GSE20303, SRP002252

Type

ChIP-seq

Organism

Homo sapiens

Released

Sept. 17, 2010

Updated

April 3, 2013

Submitter

Jesper V Olsen、 Hendrik Marks、 Falk Butter、 Michiel Vermeulen、 Filomena Matarese、 Anthony A Hyman、 Kenneth K Lee、 H C Eberl、 Hendrik Marks、 Sergei Denissov、 Matthias Mann、 Hendrik G Stunnenberg

External Links

ArrayExpress source NCBI GEO：GSE20303, SRP002252

Analysis Services

ChIP-Seq sequencing of novel interactors of H3K4me3, H3K36me3 and H3K9me3

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