Epigenome analysis of white blood cells from healthy women with and without BRCA1 mutation

·发布 2012年2月20日 ·更新 2012年6月25日

样本数

实验数

芯片平台

相关文献

实验描述

The Illumina Infinium 27k Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across approximately 27,000 CpGs in white blood cells from women with and without BRCA1 mutation. Whole blood samples were drawn from 30 BRCA1 mutation carriers (15 women with breast cancer and 15 healthy women with mean age 57.2) and 30 females without BRCA1 mutation (15 breast cancers and 15 healthy with mean age 57.1). All samples were collected between 2001 and 2008. The samples were drawn from women attending the General Faculty Hospital in Prague and the complete coding sequence, intron-exon junctions and large rearrangements for BRCA1 and BRCA2 genes were tested. Bisulphite converted DNA from the 60 samples were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2

参考文献

The dynamics and prognostic potential of DNA methylation changes at stem cell gene loci in women's cancer.

Zhuang J, Jones A, Lee SH, Ng E, Fiegl H, Zikan M, Cibula D, Sargent A, Salvesen HB, Jacobs IJ, Kitchener HC, Teschendorff AE, Widschwendter M

PMID: 22346766

芯片平台

A-GEOD-8490

Illumina HumanMethylation27 BeadChip (HumanMethylation27_270596_v.1.2)（60 例）

样本属性

age

50, 51, 52, 54, 55, 56, 57, 58, 59, 60, 61, 62, 64, 65, 67, 68, 69, 70, 80

agegrp

1, 2

beadchipid

1, 2, 3, 4, 5

bsc

10.7246589715674, 10.767194428419, 11.1489838626269, 11.3476558884014, 11.351638140969, 11.4292695062058, 11.4335740326387, 11.5805081073413, 11.5992369091635, 11.6156744677787, 11.6239553566781, 11.6621242032664, 11.6745971424239, 11.6852763952947, 11.7131123470935, 11.7242842115568, 11.7669138373497, 11.7768927967676, 11.7784347862142, 11.7867930858168, 11.8434423242749, 11.8590667839692, 11.8623809840947, 11.8709960677353, 11.8762691857226, 11.8793669830014, 11.8822720651187, 11.8914685194011, 11.8941900050262, 11.940838272323, 11.9426485922814, 11.9495346083932, 11.957538863485, 11.9601116329517, 11.9672097007853, 11.9747921247258, 11.9772889752163, 11.9927302440421, 11.9983959414842, 12.0065341631784, 12.0421180796598, 12.0540354348617, 12.0641071619788, 12.0800737416079, 12.082012285836, 12.1023523566007, 12.1384753752398, 12.1431944515214, 12.1680347775067, 12.1826339179895, 12.1834442954887, 12.1929396240795, 12.2256846439447, 12.2479491610181, 12.2506041367887, 12.3220414019169, 12.3650556709118, 12.3674833157295, 12.4153168847283, 12.7093850859133

cancer)

1, 2, 3

Organism

Homo sapiens

sample type

bisulphite converted genomic DNA from white blood cells

sentrixpos

A, B, C, D, E, F, G, H, I, J, K, L

yes)

实验信息

登记号

E-GEOD-32396

GEO 编号

GSE32396

实验类型

methylation profiling by array

物种

Homo sapiens

发布日期

2012年2月20日

更新日期

2012年6月25日

提交者

Andrew Teschendorff、 Joanna Zhuang、 Martin Widschwendter

外部链接

ArrayExpress 原始页 NCBI GEO：GSE32396

分析服务

Epigenome analysis of white blood cells from healthy women with and without BRCA1 mutation

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