DNA methylation at transcription start sites blocks TBP binding and transcription onset by RNApolII [MeDIP]

We measured the methylation levels of gene promoters of cerebral cortex of mice. We used MeDIP-array, immunoprecipitating genomic DNA with an antibody against methylated DNA and measuring methylation levels using custom arrays. The input DNA was used as control. triplicates of frontal cortex DNA pools of 3 mice