The role of PCNA ubiquitylation during unperturbed genome replication in S.pombe

To establish if PCNA ubiquitylation affects the DNA replication kinetics of specific loci, we compared rates of DNA replication across the genome between rad18+ and rad18-delta cells using the BrdU-IP sequencing method. We mapped BrdU-incorporation at 300-bp resolution and subsequent informatics analysis was performed to generate datasets of replication kinetics.