E-MTAB-10828
ChIP-seq
Saccharomyces cerevisiae S288C, Saccharomyces cerevisiae S288C
Genome-wide distribution of Rad26 and Rad1-Rad10 in relation to Mediator and RNA polymerase II
·Released June 17, 2022
12
Samples
12
Assays
Description
ChIPseq of Rad26, Rad1 and Rad10 proteins indicate their presence on chromatin in absence of UV-stress. In addition, in kin28ts mutant context, their genomic profiles change suggesting the influence of both Mediator complex and PolII transcription
Sample Attributes
Organism
Saccharomyces cerevisiae S288C
Organism part
whole organism
Growth condition
YPD media
Developmental stage
exponential phase
Genotype
MATa ura3-52 his3-Δ200 ade2-101uaa trp1-Δ63 lys2-801uag leu2-Δ1 RAD1::3HA::HIS3 MED17::13Myc::TRP1 kin28::KanMX6 CEN KIN28 LEU2, MATα ade2-1 lys2-801uag ura3-52 trp1-∆63 his3-∆200 leu2-∆ HIS3::3HA::RAD26 MED17::13Myc::TRP1 kin28::KanMX6 CEN kin28-ts16 LEU2, MATα ade2-1 lys2-801uag ura3-52 trp1-∆63 his3-∆200 leu2-∆ RAD10::3HA::HIS3 MED17::13Myc::TRP1 kin28::KanMX6 CEN KIN28 LEU2, MATα ade2-1 lys2-801uag ura3-52 trp1-∆63 his3-∆200 leu2-∆ RAD10::3HA::HIS3 MED17::13Myc::TRP1 kin28::KanMX6 CEN kin28-ts16 LEU2, MATα ade2-1 lys2-801uag ura3-52 trp1-∆63 his3-∆200 leu2-∆ HIS3::3HA::RAD26 MED17::13Myc::TRP1 kin28::KanMX6 CEN KIN28 LEU2, MATa ura3-52 his3-Δ200 ade2-101uaa trp1-Δ63 lys2-801uag leu2-Δ1 RAD1::3HA::HIS3 MED17::13Myc::TRP1 kin28::KanMX6 CEN kin28ts16 LEU2
Phenotype
wild type, temperature sensitive Kin28
Experiment Info
Accession
E-MTAB-10828
Type
ChIP-seq
Organism
Saccharomyces cerevisiae S288C, Saccharomyces cerevisiae S288C
Released
June 17, 2022
Submitter
Cyril DENBY WILKES、
Julie SOUTOURINA
External Links
ArrayExpress source
Analysis Services
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