Chromatin immunoprecipitation of TBP, NC2alpha, NC2beta, Mot1p, Spt20p, Taf1p and Rpb3p in yeast grown at 4% and shifted to 0.1% glucose

·Released Sept. 1, 2008 ·Updated May 3, 2014

Samples

Assays

Array Platforms

References

Description

ChIP-chip of TBP, NC2alpha, NC2beta, Mot1p, Spt20p, Taf1p and Rpb3p of cells grown at 4% and shifted to 0.1% glucose.

References

Cooperative action of NC2 and Mot1p to regulate TATA-binding protein function across the genome

van Werven, Folkert J.; van Bakel, Harm; van Teeffelen, Hetty A.A.M.; Altelaar, A.F. Maarten; Koerkamp, Marian Groot; Heck, Albert J.R.; Holstege, Frank C.P.; Timmers, H.Th. Marc

PMID: 18703679

Array Platforms

A-UMCU-9

UMC Utrecht S. cerevisiae 44K array, version 2.0（32 items）

Sample Attributes

BioSourceProvider

ATCC

BioSourceType

frozen_sample

CellLine

W303B

Genotype

MATalpha ade2-1 ura3-1 his3-11,15 leu2-3,112 trp1-1 can1-100, MATalpha ade2-1 ura3-1 his3-11,15 leu2-3,112 trp1-1 can1-100 Mot1-C-myc-Avitag::KANA, MATalpha ade2-1 ura3-1 his3-11,15 leu2-3,112 trp1-1 can1-100 NC2alpha-N-myc-Avitag, MATalpha ade2-1 ura3-1 his3-11,15 leu2-3,112 trp1-1 can1-100 NC2beta-N-myc-Avitag, MATalpha ade2-1 ura3-1 his3-11,15 leu2-3,112 trp1-1 can1-100 Rpb3-C-myc-Avitag::KANA, MATalpha ade2-1 ura3-1 his3-11,15 leu2-3,112 trp1-1 can1-100 Spt20-C-myc-Avitag::KANA, MATalpha ade2-1 ura3-1 his3-11,15 leu2-3,112 trp1-1 can1-100 Taf1-C-myc-Avitag::KANA, MATalpha ade2-1 ura3-1 his3-11,15 leu2-3,112 trp1-1 can1-100 TBP::Myc-N-Avitag

Immunoprecipitate

input DNA, streptavidin

Organism

Saccharomyces cerevisiae

Sex

MATalpha

StrainOrLine

Mot1-C-myc-Avitag, NC2alpha-N-myc-Avitag, NC2beta-N-myc-Avitag, Rpb3-C-myc-Avitag, Spt20-C-myc-Avitag, Taf1-C-myc-Avitag, TBP-N-myc-Avitag, Wildtype

Experiment Info

Accession

E-MTAB-21

Type

ChIP-chip by array

Organism

Saccharomyces cerevisiae

Released

Sept. 1, 2008

Updated

May 3, 2014

Submitter

Folkert J Van Werven

External Links

ArrayExpress source

Analysis Services

Chromatin immunoprecipitation of TBP, NC2alpha, NC2beta, Mot1p, Spt20p, Taf1p and Rpb3p in yeast grown at 4% and shifted to 0.1% glucose

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