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E-MTAB-3972 ERP012851 ATAC-seq Mus musculus

ATAC-seq on endocardial cells from E12.5 mouse hearts in Tbx20 knockouts and wild type counterparts

Submitted Nov. 4, 2014 ·Released June 2, 2017 ·Updated June 2, 2017
4
Samples
4
Assays
1
References
Description

We used ATAC-seq (Buenrostro et al, 2013) to identify regions of open chromatin in FACS sorted mouse endothelial cells from E12.5 hearts. Please note that the animals were injected at different dates, which resulted in different effects of the knockout, as indicated in the batch attribute of the samples. This data set is part of the study "Endocardial Tbx20 is essential for mesenchymal and myocardial cell movements required for cardiac septation". Peaks were called using HOMER (-gsize 1.87e9 -region -tbp 1). Replicate 1: -fdr 1e-8, replicate 2: default parameters. Buenrostro JD, Giresi PG, Zaba LC, Chang HY, Greenleaf WJ. 2013. Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nat Methods 10: 12138.

References
Probing chromatin landscape reveals roles of endocardial TBX20 in septation
Boogerd CJ, Aneas I, Sakabe N, Dirschinger RJ, Cheng QJ, Zhou B, Chen J, Nobrega MA, Evans SM
PMID: 27348591
Sample Attributes
batch
2014-09-26, 2014-11-05
cell type
endocardial cell
developmental stage
embryonic day 12.5
genotype
Tbx20 knockout, wild type genotype
organism
Mus musculus
organism part
heart
replicate
1, 2
strain
Black Swiss mouse strain
Experiment Info
Accession
E-MTAB-3972
GEO ID
ERP012851
Type
ATAC-seq
Organism
Mus musculus
Submitted
Nov. 4, 2014
Released
June 2, 2017
Updated
June 2, 2017
Submitter
Kees j Boogerd、 Noboru Sakabe
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