RHNO1 (RAD9-HUS1-RAD1 interacting nuclear orphan 1)

Gene Basic Information

Gene Basic Information

symbol:
RHNO1
locus group:
protein-coding gene
location:
12p13.33
gene_family:
alias symbol:
HKMT1188|MGC13204|RHINO
alias name:
Rad9, Rad1, Hus1 interacting nucle…
entrez id:
83695
ensembl gene id:
ENSG00000171792
ucsc gene id:
uc031qfr.2
refseq accession:
NM_031465
hgnc_id:
HGNC:28206
approved reserved:
2006-01-17

Chromosome Location

Chromosome Location

12p13.33

Gene Summary

Gene Summary

RHNO1（也称为RHINO或RAD9-HUS1-RAD1 interacting nuclear orphan 1）是一个与DNA损伤应答和细胞周期调控密切相关的基因。它属于RAD9-HUS1-RAD1（9-1-1）复合体相互作用蛋白家族，该家族成员通常参与维持基因组稳定性并在DNA损伤修复中起关键作用。RHNO1的主要功能是在DNA复制应激或损伤时被ATR激酶磷酸化，进而招募到DNA损伤位点，促进ATR-CHK1信号通路的激活，这对细胞周期检查点的调控和DNA修复至关重要。RHNO1的作用位点主要集中在细胞核内，特别是在DNA损伤引发的复制叉停滞处。突变或功能缺失可能导致DNA损伤应答缺陷，增加基因组不稳定性，进而与癌症发生发展相关，例如某些乳腺癌和卵巢癌中观察到RHNO1表达异常。RHNO1过表达可能增强细胞对DNA损伤因子的抵抗，但过度激活也可能干扰正常细胞周期进程；而降低表达则会使细胞对复制应激更敏感，导致染色体断裂和细胞凋亡增加。RHNO1与9-1-1复合体的相互作用是其功能的核心，该复合体作为滑动夹子稳定停滞的复制叉。基因家族共性包括：均含有保守的PCNA结合模体，参与DNA代谢过程，并在细胞周期检查点调控中协同作用。研究表明，RHNO1还与同源重组修复（HR）通路相关蛋白如BRCA1/2存在功能联系，提示其在癌症治疗靶点开发中的潜在价值。

ChineseEnglish

None

OMIM entry for RHNO1     Wikipedia entry for RHNO1

Gene Sequence

Gene Sequence

Nucleotide sequence of RHNO1:[NCBI]
Loading Gene Browser...

Encoded Protein

Encoded Protein

Protein Sequence
1MPPRKKRRQP SQKAPLLFHQ QPLEGPKHSC ASTQLPITHT
41RQVPSKPIDH STITSWVSPD FDTAAGSLFP AYQKHQNRAR
81 HSSRKPTTS KFPHLTFESP QSSSSETLGI PLIRECPSES
121EKDVSRRPLV PVLSPQSCGN MSVQALQSLP YVFIPPDIQT
161P ESSSVKEE LIPQDQKENS LLSCTLHTGT PNSPEPGPVL
201VKDTPEDKYG IKVTWRRRQH LLAYLRERGK LSRSQFLVKS
结构预测来自 AlphaFold DB（UniProt: Q9BSD3），颜色表示 pLDDT 置信度（深蓝高、黄橙低）。

Gene Variants

Gene Variants

SNP variants of RHNO1:           Showing partial SNPs
rs1860433       rs1860434       rs3829865       rs4766002       rs4766003       rs5796032       rs7138232       rs7958624       rs7969183       rs10848719       rs11062396       rs11427501       rs12425068       rs12582464       rs12818029       rs28918668       rs28990677      

Gene Expression

Gene Expression

Tissue expression of RHNO1:    [UniProt]

Gene expression across tissues

Gene Primers

Gene Primers

Forward Primer
Forward Tm
Reverse Primer
Reverse Tm
Score
GTTCCACCAACAACCACTG
59
ATCAGGTGATACCTGTCGAG
59
CTACACAGCTTCCCATCAC
58
AGGTGATACCCAGGAAGTG
58
CTACACAGCTTCCCATCAC
58
AGGTGATACCCAGGAAGTG
58
GCTTACCTTATGTGTTCATTCC
58
TCCTTCACAGACGATGACTC
59
GCTTACCTTATGTGTTCATTCC
58
TCCTTCACAGACGATGACTC
59
CTACACAGCTTCCCATCAC
58
AGGTGATACCCAGGAAGTG
58
CTACACAGCTTCCCATCAC
58
AGGTGATACCCAGGAAGTG
58
TGCTACATTCTCAAGAACCAG
58
TGGTGTTTCTGGTAGGCTG
60
CTACACAGCTTCCCATCAC
58
AGGTGATACCCAGGAAGTG
58
TCACTTCCTGGGCATTCTG
60
CAAAGTGCTGGGATTACAGG
59

Transcription Factors

Transcription Factors

      No data available

Gene Ontology

Subcellular localization of RHNO1 (and its protein):

[UniProt]     [GenomeNet]

" d="M482.414,245.296c3.539,4.293,4.455,10.009,0.202,11 c-4.244,0.996-4.983-10.983-8.293-8.438c-5.271,4.08,9.834,12.271,5.144,17.287c-3.717,3.607-6.172-5.75-10.839-1.976 c-4.673,3.776,6.781,7.299,2.831,11.326c-4.354,4.045-6.979-1.449-9.837-5.517c-1.193-1.742-2.059-3.851-3.595-2.748 c-1.516,1.078-1.854,1.795-0.938,3.666c2.374,4.854,9.235,10.119,5.156,12.535c-5.636,3.346-5.044-8.871-9.426-7.574 c-4.388,1.291,2.557,10.66-1.245,11.141c-4.089,0.545-3.483-10.239-6.979-8.575c-2.522,1.206-0.929,3.071-0.938,4.899 c0.004,1.32-0.964,3.6-2.372,4.062c-3.593,1.171-8.544-1.065-10.251-3.59c-6.04-8.93,0.396-15.997,4.639-7.015 c3.023,4.642,5.182,0.834,2.839-2.219c-1.032-1.354-4.309-5.901-0.781-7.252c2.904-1.113,4.271,1.941,5.985,4.592 c2.61,4.016,5.485,0.117,3.031-3.414c-1.828-2.633-2.74-3.803,3.156-7.42c6.405-4.369,6.52,3.869,10.077,0.646 c2.309-1.832-4.783-5.149,0.06-8.995c2.896-2.293,5.18,6.207,7.961,3.516c3.523-2.737-7.717-7.369,0.117-11.736 C473.413,240.77,480.519,242.891,482.414,245.296z"/> Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
  • plasma membrane
  • cytoplasm
  • extracellular
  • golgi
  • vesicle
  • cytoskeleton
  • endoplasmic reticulum
  • nucleus
  • endosome
  • lysosome
  • mitochondrion

Gene Ontology (GO) terms for RHNO1:

GO ID
Protein
Source DB
GO:0000077
F5H0U1 (UniProtKB)
IEA
GO:0005634
F5H0U1 (UniProtKB)
IEA
GO:0071479
F5H0U1 (UniProtKB)
IEA
GO:0000077
F5H580 (UniProtKB)
IEA
GO:0005634
F5H580 (UniProtKB)
IEA
GO:0071479
F5H580 (UniProtKB)
IEA
GO:0000077
F5H795 (UniProtKB)
IEA
GO:0005634
F5H795 (UniProtKB)
IEA
GO:0071479
F5H795 (UniProtKB)
IEA
GO:0000077
F5H7S2 (UniProtKB)
IEA
GO:0005634
F5H7S2 (UniProtKB)
IEA
GO:0071479
F5H7S2 (UniProtKB)
IEA
GO:0000077
Q9BSD3 (UniProtKB)
IMP
GO:0000725
Q9BSD3 (UniProtKB)
IMP
GO:0005515
Q9BSD3 (UniProtKB)
IPI
GO:0005515
Q9BSD3 (UniProtKB)
IPI
GO:0005515
Q9BSD3 (UniProtKB)
IPI
GO:0005515
Q9BSD3 (UniProtKB)
IPI
GO:0005515
Q9BSD3 (UniProtKB)
IPI
GO:0005515
Q9BSD3 (UniProtKB)
IPI
GO:0005515
Q9BSD3 (UniProtKB)
IPI
GO:0005515
Q9BSD3 (UniProtKB)
IPI
GO:0005634
Q9BSD3 (UniProtKB)
IDA
GO:0005654
Q9BSD3 (UniProtKB)
TAS
GO:0005654
Q9BSD3 (UniProtKB)
TAS
GO:0005654
Q9BSD3 (UniProtKB)
TAS
GO:0005654
Q9BSD3 (UniProtKB)
TAS
GO:0005654
Q9BSD3 (UniProtKB)
TAS
GO:0005654
Q9BSD3 (UniProtKB)
TAS
GO:0005654
Q9BSD3 (UniProtKB)
TAS
GO:0005654
Q9BSD3 (UniProtKB)
TAS
GO:0005654
Q9BSD3 (UniProtKB)
TAS
GO:0005654
Q9BSD3 (UniProtKB)
TAS
GO:0005694
Q9BSD3 (UniProtKB)
IDA
GO:0006260
Q9BSD3 (UniProtKB)
TAS
GO:0034644
Q9BSD3 (UniProtKB)
IDA
GO:0070318
Q9BSD3 (UniProtKB)
IMP
GO:0071479
Q9BSD3 (UniProtKB)
IDA
GO:1901796
Q9BSD3 (UniProtKB)
TAS

Related microRNA

Related microRNA

microRNAs potentially regulating RHNO1:     

Gene Interactions

Gene Interactions

BioGrid
IntAct
mentha
Loading…
Interacting Gene Interaction Source/Score

Gene Pathways

Gene Pathways

Related Diseases

Related Diseases

Disease Score NofPmids NofSnps Source
Disease Score NofPmids NofSnps Source
Breast Carcinoma 0.000542884 2 0 BeFree
Malignant neoplasm of breast 0.000542884 2 0 BeFree
Carcinogenesis 0.000271442 1 0 BeFree

Related Publications

Related Publications

A RHNO1-ATR/Chk1 positive feedback loop sustains the DNA replication stress response.
Jirapongwattana N, Conover CM, Trujillo Jaramillo C, Chiruvella KK, Her J, Ghosal G, Bunting SF, Ramsden DA, Karpf AR bioRxiv 2026-06-12
Candidate DNA repair susceptibility genes identified by exome sequencing in high-risk pancreatic cancer.
Smith Alyssa L, Alirezaie Najmeh, Connor Ashton, Chan-Seng-Yue Michelle, Grant Robert, Selander Iris, Bascuñana Claire, Borgida Ayelet, Hall Anita, Whelan Thomas, Holter Spring, McPherson Treasa, Cleary Sean, Petersen Gloria M, Omeroglu Atilla, Saloustros Emmanouil, McPherson John, Stein Lincoln D, Foulkes William D, Majewski Jacek, Gallinger Steven, Zogopoulos George Cancer Lett IF: 11.8 2016-05-03

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