Femtomolar Zn(II) affinity of minimal zinc hook peptides--a promising small tag for protein engineering.

Chemical communications (Cambridge, England) ·Vol. 49 ·No. 13 ·2013-08-14

Kochańczyk Tomasz, Jakimowicz Piotr, Krężel Artur

Abstract

The minimal zinc hook peptide of Rad50 and its alanine mutants form highly stable Zn(II) complexes. These peptides were successfully used as a small, efficient tag for reversible Zn(II)-mediated protein homodimerization. The high stability, its biological consequences and potential applications in protein engineering are discussed.

Article Info

Journal

Chemical communications (Cambridge, England)

Abbr.

Chem Commun (Camb)

ISSN

1359-7345

DOI

10.1039/c2cc38174e

Published

2013-08-14

Indexed

2013-01-18

Updated

2013-11-21

Language

English

Country/Region

England

NLM ID

9610838

External Links

PubMed source DOI: 10.1039/c2cc38174e View journal details

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Femtomolar Zn(II) affinity of minimal zinc hook peptides--a promising small tag for protein engineering.

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