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Assay of centromere function using a human artificial chromosome.

Masumoto(H),Ikeno(M),Nakano(M),Okazaki(T),Gr… Chromosoma 1999-03-11

In order to define a functional human centromere sequence, an artificial chromosome was constructed as a reproducible DN...

GFP tagging reveals human Polo-like kinase 1 at the kinetochore/centromere regi…

Arnaud(L),Pines(J),Nigg(E A) Chromosoma 1999-03-11

Polo-like kinases (Plks) have been implicated in various aspects of M-phase progression in organisms ranging from yeast ...

Characterization of mouse ubiquitin-like SMT3A and SMT3B cDNAs and gene/pseudog…

Chen(A),Mannen(H),Li(S S) Biochem Mol Biol Int 1999-03-25

Mouse SMT3A and SMT3B cDNAs encoding ubiquitin-like proteins of 110 and 95 amino acids, respectively, were isolated and ...

Human artificial chromosomes generated by modification of a yeast artificial ch…

Henning(K A),Novotny(E A),Compton(S T),Guan(… Proc Natl Acad Sci U S A 1999-03-16

A human artificial chromosome (HAC) vector was constructed from a 1-Mb yeast artificial chromosome (YAC) that was select...

Single base discrimination of CENP-B repeats on mouse and human Chromosomes wit…

Chen(C),Hong(Y K),Ontiveros(S D),Egholm(M),S… Mamm Genome 1999-03-01

The satellite repeat structure of the mammalian centromere contains the CENP-B protein binding site. Using the peptide n...

Visualization of prekinetochore locus on the centromeric region of highly exten…

Sugimoto(K),Tsutsui(M),AuCoin(D),Vig(B K) Chromosome Res 1999-06-03

Kinetochore is morphologically defined as a trilaminated, highly differentiated structure at the primary constriction of...

Human nuclear matrix contains a protein complex which can specifically recogniz…

Enukashvily(N I),Lobov(I B),Podgornaya(O I) Biochemistry (Mosc) 1999-07-19

A nuclear matrix protein complex has been found which specifically binds to alpha-satellite DNA as revealed by gel shift...

Transmission of a fully functional human neocentromere through three generation…

Tyler-Smith(C),Gimelli(G),Giglio(S),Floridia… Am J Hum Genet 1999-05-20

An unusual Y chromosome with a primary constriction inside the long-arm heterochromatin was found in the amniocytes of a...

Use of PCR to screen for promoter elements in genomic DNA library clones.

Poppe(M),Hahm(B),Eickelbaum(W),Arand(M),Pawe… Biotechniques 1999-08-13

We report a modified PCR strategy to screen for promoter elements of genes of interest that is based upon consecutive ro...

The cloning and analysis of LEK1 identifies variations in the LEK/centromere pr…

Goodwin(R L),Pabón-Peña(L M),Foster(G C),Bad… J Biol Chem 1999-07-15

We report the cloning of a novel murine cDNA, LEK1, that is related to human CENP-F and mitosin and more distantly to ch...

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